Journal: Cancer Science

Article Title: Pigpen, a nuclear coiled body component protein, is involved in angiogenesis

doi: 10.1111/j.1349-7006.2010.01494.x

Figure Lengend Snippet: Immunopreciptation by anti‐puromycin‐insensitive leucyl‐specific aminopeptidase (PILSAP) Ab followed by western blotting of pigpen and PILSAP (a,b) and immunocytochemistry of pigpen (green) and PILSAP (red) (c) in MSS31 cells treated with vehicle, 50 ng/mL vascular endothelial growth factor (VEGF), or 20 ng/mL basic fibroblast growth factor (bFGF) for 20 min. Nuclei were counterstained with TO‐PRO‐3 (blue). Bars = 20 μm.

Article Snippet: The cell extract (500 μg) was incubated with 10 μg antimouse PILSAP goat IgG (anti‐mPILSAP Ab; R&D Systems, Minneapolis, MN, USA) for 13 h at 4°C.

Techniques: Western Blot, Immunocytochemistry

Journal: Cancer Science

Article Title: Pigpen, a nuclear coiled body component protein, is involved in angiogenesis

doi: 10.1111/j.1349-7006.2010.01494.x

Figure Lengend Snippet: Western blot of pigpen in scr‐ or siPigpen‐transfected MSS31, MS1, and Lewis lung carcinoma (LLC) cells (a). Western blot of pigpen and PILSAP in MSS31 transfected with scr‐ or siPigpen, siPigpen‐A, siPigpen‐B, or nontargeting siRNA (NC siRNA) as a negative control for siPigpen‐A and ‐B (b). Equal loading was confirmed by β‐actin.

Article Snippet: The cell extract (500 μg) was incubated with 10 μg antimouse PILSAP goat IgG (anti‐mPILSAP Ab; R&D Systems, Minneapolis, MN, USA) for 13 h at 4°C.

Techniques: Western Blot, Transfection, Negative Control